Journal: Cell Reports

Article Title: Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer

doi: 10.1016/j.celrep.2018.11.069

Figure Lengend Snippet:

Article Snippet: Single cells suspensions were embedded in growth factor reduced Matrigel (Corning/BD, 354230) or BME-2 (AMSBIO, 3533-010-02) and plated a 40 μL drop/well (1,000-3,000 cells/drop) in the center of 96-well optical bottom plates.

Techniques: RNAscope, Recombinant, Concentration Assay, Microarray, Expressing, Software

Journal: Frontiers in Medicine

Article Title: Identification and Validation of Novel Serum Autoantibodies Biomarkers for Staging Liver Fibrosis in Patients With Chronic Hepatitis B

doi: 10.3389/fmed.2021.807087

Figure Lengend Snippet: Microarray detection of serum samples. (A) Scan images of a representative array; (B) Design of the protein microarray; Microarray detection with sera from healthy control (C) and chronic hepatitis B patients with S0-1 (D) , S2-3 (E) and S4 (F) . IgG was serial diluted to constructed standard curve for each teat.ACY1/HSPA6/CENPF-2 was double diluted.

Article Snippet: Antigenic proteins CENPF (8 μg/mL), ACY1 (4 μg/mL) were incubated in 96-well-microplates (Corning, Corning, NY) with 100 μL coating buffer (0.05 M carbonate/bicarbonate, pH 9.6) in each well.

Techniques: Microarray, Construct

Journal: Frontiers in Medicine

Article Title: Identification and Validation of Novel Serum Autoantibodies Biomarkers for Staging Liver Fibrosis in Patients With Chronic Hepatitis B

doi: 10.3389/fmed.2021.807087

Figure Lengend Snippet: ELISA detection and validation of serum relative levels of CENPF and ACY1 autoantibodies. Scatter diagram of the relative levels of autoantibodies to CENPF (A) and ACY1 (B) in patients with different stages of liver fibrosis. Line, median with interquartile range. (C) The standard curve of a representative ELISA plate for the quantification of CENPF autoantibodies, which was generated using the four-parameter logistic regression model. (D,E) ELISA showing the specificity of CENPF and ACY1 proteins detected by in-house anti-CENPF antibody (43 ug/ml) and commercial anti-ACY1 antibody (abnova#H00000095-A01); (F) Western blots showing reactivity of sera with various levels of autoantibody obtained by ELISA to recombinant protein CENPF or ACY1. The lane with “+” indicates the corresponding reference antibodies used as a positive control; lanes a–c, three sera with OD values of 1.18, 0.56 and 0.27 detected by the same CENPF ELISA plate; lanes d–f, three sera with OD values of 1.04, 0.69, and 0.45 detected by the same ACY1 ELISA plate; M, PageRuler prestained protein adder (Thermo Fisher Scientific). * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Antigenic proteins CENPF (8 μg/mL), ACY1 (4 μg/mL) were incubated in 96-well-microplates (Corning, Corning, NY) with 100 μL coating buffer (0.05 M carbonate/bicarbonate, pH 9.6) in each well.

Techniques: Enzyme-linked Immunosorbent Assay, Generated, Western Blot, Recombinant, Positive Control

Journal: Frontiers in Medicine

Article Title: Identification and Validation of Novel Serum Autoantibodies Biomarkers for Staging Liver Fibrosis in Patients With Chronic Hepatitis B

doi: 10.3389/fmed.2021.807087

Figure Lengend Snippet: The diagnostic values of the selected autoantibodies in different stages of liver fibrosis. Receiver operating characteristic curve analysis (A–E) and comparison of the positivity rate (F–J) of autoantibodies against CENPF and ACY1. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Antigenic proteins CENPF (8 μg/mL), ACY1 (4 μg/mL) were incubated in 96-well-microplates (Corning, Corning, NY) with 100 μL coating buffer (0.05 M carbonate/bicarbonate, pH 9.6) in each well.

Techniques: Diagnostic Assay

Journal: Frontiers in Medicine

Article Title: Identification and Validation of Novel Serum Autoantibodies Biomarkers for Staging Liver Fibrosis in Patients With Chronic Hepatitis B

doi: 10.3389/fmed.2021.807087

Figure Lengend Snippet: The diagnostic performance of CENPF and ACY1 autoantibodies for staging liver fibrosis tested by ELISA.

Article Snippet: Antigenic proteins CENPF (8 μg/mL), ACY1 (4 μg/mL) were incubated in 96-well-microplates (Corning, Corning, NY) with 100 μL coating buffer (0.05 M carbonate/bicarbonate, pH 9.6) in each well.

Techniques: Diagnostic Assay, Enzyme-linked Immunosorbent Assay